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Ins and outs of digital electron microscopy

✍ Scribed by Koeck, Philip J.B.

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
97 KB
Volume
49
Category
Article
ISSN
1059-910X

No coin nor oath required. For personal study only.

✦ Synopsis

In order to do any sort of computer processing of the images produced by an electron microscope they have to be digitized. Therefore, any form of microscopy that involves computer processing has to be considered as digital microscopy independently of whether the original images were recorded on film and scanned or recorded directly on a digital medium such as the charge coupled device (CCD). As soon as one digitizes images, one encounters a range of effects and artefacts specific to the digital world such as aliasing, quantization errors, and ambiguities in the modulation transfer function. Even a quick discussion of these effects makes it clear that what comes out of a digitizer is not related in a simple way to what goes in. After introducing the commonly used recording media, I will discuss the most important effects of digitization and then compare the limitations and advantages of the different recording media for various applications.

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