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Initiation of phage φ29 DNA replication by mutants with deletions at the carboxyl end of the terminal protein

✍ Scribed by Zaballos, Angel; Salas, Margarita; Mellado, Rafael P.


Book ID
122877294
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
714 KB
Volume
43
Category
Article
ISSN
0378-1119

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In vitro initiation of bacteriophage Φ29
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Cell-free extracts prepared from phi 29 and M2-infected Bacillus subtilis cells catalyse the formation of complexes between terminal protein and [alpha-32P]-dAMP in the presence of [alpha-32P]-dATP, MgCl2, ATP, and phage DNA with terminal protein covalently linked at both the 5'ends. The complex for