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Cloning and expression of gene 2, required for the protein-primed initiation of the Bacillus subtilis phage φ29 DNA replication

✍ Scribed by Blanco, Luis; García, Juan A.; Salas, Margarita

Book ID
123344953
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
794 KB
Volume
29
Category
Article
ISSN
0378-1119

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✍ Matsumoto, Kouji ;Saito, Toshiyuki ;Hirokawa, Hideo 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 966 KB

Cell-free extracts prepared from phi 29 and M2-infected Bacillus subtilis cells catalyse the formation of complexes between terminal protein and [alpha-32P]-dAMP in the presence of [alpha-32P]-dATP, MgCl2, ATP, and phage DNA with terminal protein covalently linked at both the 5'ends. The complex for