Inhibitory effects of anti-tumor platinum compounds on DNA, RNA and protein syntheses in mammalian cells in vitro

✍ Scribed by Harold C. Harder; Barnett Rosenberg

• Publisher: John Wiley and Sons
• Year: 1970
• Tongue: French
• Weight: 681 KB
• Volume: 6
• Category: Article
• ISSN: 0020-7136
• DOI: 10.1002/ijc.2910060207

✦ Synopsis

The inhibitory effects of anti-tumor, bacterial filament forming platinum compounds, Cis-Pt ( I I ) ( NH,) ,ClZ ( A ) , Cis-Pt ( I V ) ( NH,) ,C14 ( B ) , and Pt (11) ( NH,) , (CH,) , CI, ( C ) on DNA, RNA and protein syntheses was measured by the incorporation of 3Hthymidine, 3H-uridine, and 3H-L-leucine, respectively, into an acid-insoluble polymer in human amnion A V 3 cells. Compound A , the most effective tumor-inhibiting platinum compound, was shown to selectively inhibit D N A synthesis below 5pM and to inhibit 3H-thymidine incorporation more rapidly than 3H-uridine or 3H-leucine incorporation at 2SpM. Like A, compounds B and C were also shown to inhibit all three processes after a 24-hour period of treatment at 2SpM. A correlation was established between the relative anti-tumor effectiveness of compounds A , B, and C and the extent of their inhibitory effects. On the other hand, two non-tumor-inhibiting platinum compounds, [Pt (II) (NH3)JCI2 ( D ) and Trans-Pt ( I V ) (NH,),CI, ( E ) had no inhibitory effects,