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Inhibitory effect of serum from rats administered with coffee on the proliferation and invasion of rat ascites hepatoma cells

✍ Scribed by Yutaka Miura; Tamio Furuse; Kazumi Yagasaki

Book ID: 110256201
Publisher: Springer
Year: 1997
Tongue: English
Weight: 54 KB
Volume: 25
Category: Article
ISSN: 0920-9069
DOI: 10.1023/a:1007915917201

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## Abstract A neutral protease was extracted from rat ascites hepatoma cells and effectively purified by fractionation with ammonium sulfate followed by chromatography using TEAE‐cellulose and hydroxylapatite in that order. The protease eluted in 120 mM phosphate buffer (pH 6.8) on hydroxylapatite,