Inhibitory effect of danazol on melanogenesis in mouse B16 melanoma cells
โ Scribed by Te-Sheng Chang; Jia-Jhen Lin
- Publisher
- Springer
- Year
- 2010
- Tongue
- English
- Weight
- 469 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0253-6269
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## Abstract A permanent cell line C~2~M of mouse melanoma B16 was highly melanized in a modified Eagle's MEM supplemented with 10% calf serum, when the medium contained 1 mM galactose and 10 mM pyruvate instead of 5.5 mM glucose. The activity of the key enzyme for melanogenesis, tyrosinase (EC 1.14
The effect of theophylline, an inhibitor of phosphodiesterase, on tyrosine (Tyr) transport across cell membrane was studied using cultured B-16 mouse melanoma cells. 1.5 mM Theophylline in culture medium increased Tyr uptake velocity in linear fashion up to 36 h, after which it reached a plateau at