Effect of TunisianCapparis spinosaL. extract on melanogenesis in B16 murine melanoma cells
โ Scribed by Kyoko Matsuyama; Myra O. Villareal; Abdelfatteh El Omri; Junkyu Han; Mohamed Elyes Kchouk; Hiroko Isoda
- Publisher
- Springer Japan
- Year
- 2009
- Tongue
- Japanese
- Weight
- 215 KB
- Volume
- 63
- Category
- Article
- ISSN
- 1340-3443
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To elucidate the interaction between melanoma and its matrix, we cultured B16 murine melanoma cells on and in type I collagen gel and evaluated specified functions of melanoma cells; tyrosinase activity and melanin-synthesizing capacity. Proliferation of cells cultured in these environments was mark
## Abstract A permanent cell line C~2~M of mouse melanoma B16 was highly melanized in a modified Eagle's MEM supplemented with 10% calf serum, when the medium contained 1 mM galactose and 10 mM pyruvate instead of 5.5 mM glucose. The activity of the key enzyme for melanogenesis, tyrosinase (EC 1.14