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Inhibition of the substrate cycle glucose: Glucose 6-phosphate by physiological concentrations of fructose in perfued rat liver

✍ Scribed by Dallas G. Clark; Gerald B. Storer; David L. Topping

Book ID
119406888
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
341 KB
Volume
93
Category
Article
ISSN
0006-291X

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A method for the determination of glucose-6-phosphatase activity in rat liver with [U-14C]glucose 6-phosphate as substrate
✍ S.A. Kitcher; K. Siddle; J.P. Luzio πŸ“‚ Article πŸ“… 1978 πŸ› Elsevier Science 🌐 English βš– 414 KB

A method is described for measuring the activity of glucose-6-phosphatase (EC 3.1.3.9) in rat liver. [U-W]Glucose 6-phosphate, as substrate, is converted by the enzyme to [Ylglucose and inorganic phosphate. The addition of ZnSO, and Ba(OH), at the end of the reaction precipitates phosphate and the u