Inhibition of the growth of human melanoma xenografts in nude mice by human tumor-specific cytotoxic T-cells

✍ Scribed by Nancy J. Crowley; Craig L. Slingluff Jr.; Carol E. Vervaert; Timothy L. Darrow; Hilliard F. Seigler

• Publisher: John Wiley and Sons
• Year: 1990
• Tongue: English
• Weight: 538 KB
• Volume: 43
• Category: Article
• ISSN: 0022-4790
• DOI: 10.1002/jso.2930430202

✦ Synopsis

Melanoma-specific T-cells (CTLs) are specifically cytotoxic for autologous tumor, when assayed in vitro. To examine their effectiveness in vivo, we tested the ability of these human T-cells to inhibit growth of human melanoma xenografts by using a Winn assay. Nude mice receiving specific CTLs (n = 10) demonstrated a dramatic inhibition of tumor growth. All treated mice were tumor-free at day SO and nine remained tumor-free at day 6.5, vs. control mice (n= 10) with average tumor volumes of 321 mm3 and 808 mm3, respectively. To control for the possibility that a non-specific response to the human T-cells could inhibit tumor growth, an additional group received allospecific CTLs. There was no inhibition of tumor growth in this group (n = 8), with the average tumor volume of 2,768 mm' at day 40 vs. 1,882 mm3 in the control group (n= 10). We conclude that these tumor-specific CTLs can inhibit tumor growth in vivo and may prove useful in the adoptive immunotherapy of melanoma.