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Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences

✍ Scribed by Lusky, Monika; Botchan, Michael


Book ID
109724000
Publisher
Nature Publishing Group
Year
1981
Tongue
English
Weight
453 KB
Volume
293
Category
Article
ISSN
0028-0836

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## Abstract Specific antigens at the surface of SV40‐transformed hamster cells were demonstrated by the in vitro colony inhibition and immunofluorescence tests. Antisera were prepared by inoculating hamsters either with purified SV40 or with human or marmoset SV40‐transformed cells. The inhibition

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## Abstract In situ hybridization has been used as a quantitative method to study the replication of SV40 during lytic infection. Various parameters have been defined such as fixation and denaturation of cytological preparations, concentration of complementary (^3^H)‐cRNA and volume of buffer used