𝔖 Bobbio Scriptorium
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Inhibition of rabbit sperm acrosomal enzymes by gossypol

✍ Scribed by Y. Y. Yuan; Q. X. Shi; P. N. Srivastava

Book ID
102951626
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
518 KB
Volume
40
Category
Article
ISSN
1040-452X

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✦ Synopsis

Abstract

The effect of gossypol on the activities of 10 acrosomal enzymes of the rabbit sperm was evaluated. Acrosin, Azocoll proteinase, neuraminidase, and arylsulfatase were significantly inhibited or completely inactivated by 12–76 μM gossypol. Hyaluronidase, β‐glucuronidase, and acid phosphatase were inhibited only at a higher concentration of gossypol (380 μM). Phospholipase C, alkaline phosphatase, and β‐N‐Acetyl glucosaminidase were not inhibited even at 380 μM gossypol. Gossypol was found to be a noncompetitive inhibitor of arylsulfatase with a Ki of 120 μM. The inhibition was reversible and dose‐dependent. As the acrosomal enzymes were more sensitive to the inhibition by gossypol compared to sperm enzymes involved in glycolysis or energy production, these assays may serve as a more reliable indicator for monitoring the occurence of gossypol‐induced sterility. © 1995 Wiley‐Liss, Inc.

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