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Inhibition of postconfluent focus production in cultures of MCF-7 human breast cancer cells by 2,3,7,8-tetrachlorodibenzo-p-dioxin

✍ Scribed by John F. Gierthy; David W. Lincoln


Publisher
Springer US
Year
1988
Tongue
English
Weight
566 KB
Volume
12
Category
Article
ISSN
0167-6806

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Filtration assay for quantitation of 2,3
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A rapid and sensitive filtration assay for quantitating the specific binding of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) to whole cells in culture is described. Cell monolayers are incubated with [3H]TCDD in the presence or absence of excess unlabeled ligand, detached from the culture dish with tr