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Inhibition of polymerase chain reaction for the detection of Escherichia coli O157:H7 and Salmonella enterica on walnut kernels

โœ Scribed by Ganz, Kyle; Gill, Alex

Book ID
122395324
Publisher
Elsevier Science
Year
2013
Tongue
English
Weight
355 KB
Volume
35
Category
Article
ISSN
0740-0020

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โœ Nathalie Y. Fortin; Ashok Mulchandani; Wilfred Chen ๐Ÿ“‚ Article ๐Ÿ“… 2001 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 87 KB

Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t