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Inhibition of maturation and metabolism in rat oocytes by cyclic AMP

✍ Scribed by Magnusson, Claes ;Hillensjö, Torbjörn

Publisher
John Wiley and Sons
Year
1977
Tongue
English
Weight
492 KB
Volume
201
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

It is known that dibutyryl cyclic AMP (dbcAMP) and theophylline inhibit the spontaneous maturation of isolated mouse oocytes. The present study demonstrates that dbcAMP (0.01 – 1.0 mM) as well as cyclic AMP (cAMP, 10 mM) and a phosphodiesterase inhibitor (IBMX, 0.01 – 1.0 mM) prevent spontaneous maturation of isolated rat oocytes. As reported earlier an increase in oxygen consumption by the oocyte was found following maturation. When the oocytes were cultured in the presence of dbcAMP or cAMP no change in respiration occurred during culture.

These results argue against the theory that cAMP acts as a direct mediator of the action of luteinizing hormone (LH) on oocyte maturation. Furthermore they suggest that changes in oocyte energy metabolism are closely related to the maturation process.

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