Inhibition of fertilization in cattle by passive immunization with anti-zona pellucida serum
β Scribed by Tsunoda, Y. ;Soma, T. ;Sugie, T.
- Book ID
- 111662799
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1981
- Weight
- 375 KB
- Volume
- 4
- Category
- Article
- ISSN
- 0148-7280
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Bovine zonae were solubilized by heating t o 80Β°C in buffered saline. On injection of female rabbits with this solution, zona-specific antisera were produced that could be assayed on bovine eggs in vitro by immunofluorescence, coating, and inhibition of sperm-binding. When the latter was complete, t
## Abstract The fertilization __in vitro__ of mouse eggs with intact zonae pellucidae by mouse cauda epididymal spermatozoa was inhibited in a concentrationβdependent fashion by 3βquinuclidinyl benzilate (QNB), normally used as a specific antagonist for the muscarinic class of cholinergic receptors