Immunization of female rabbits with heat-solubilized bovine zonae: Production of anti-zona antibody and inhibition of fertility
✍ Scribed by Gwatkin, R. B. L. ;Williams, D. T.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1978
- Weight
- 446 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0148-7280
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✦ Synopsis
Bovine zonae were solubilized by heating t o 80°C in buffered saline. On injection of female rabbits with this solution, zona-specific antisera were produced that could be assayed on bovine eggs in vitro by immunofluorescence, coating, and inhibition of sperm-binding. When the latter was complete, the rabbits were found to be infertile. Immunofluorescence assays showed that the rabbit anti-bovine zona serum reacted as strongly with rabbit and rhesus monkey zonae as with homologous zonae. Strong cross-reaction was also observed with marmoset and dog zonae, but with hamster zonae the reaction was relatively weak.