The receptor for epidermal growth factor (EGF) is a glycosylated transmembrane phosphoprotein that exhibits EGF-stimulable protein tyrosine kinase activity. On EGF stimulation, the receptor undergoes a self-phosphorylation reaction at tyrosine residues located primarily in the extreme carboxyl-termi
Inhibition of epidermal growth factor-induced phosphorylation by trifluoperazine
✍ Scribed by Alonzo H. Ross; Caroline Damsky; Paul D. Phillips; Frances Hwang; Patricia Vance
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 943 KB
- Volume
- 124
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
✦ Synopsis
Murine monoclonal antibodies
Ascites from hybridoma EGF-R1, which contains monoclonal antibodies directed against the EGF receptor (Waterfield et al., 1982), was a generous gift from Dr. Peter Goodfellow. We routinely used 3 p1 per sample, which was enough to saturate our immunoprecipitation
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