Inhibition of cation-induced DNA condensation by intercalating dyes
β Scribed by Jonathan Widom; Robert L. Baldwin
- Book ID
- 102762525
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1983
- Tongue
- English
- Weight
- 625 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
Synopsis
Several intercalating dyes are shown to inhibit the cation-induced condensation of A-DNA when Co3+(NH3)6 is the condensing agent. The dyes that have been studied are ethidium, propidium, proflavin, quinacrine, and actinomycin D. Earlier work has shown that intercalating dyes inhibit $-DNA condensation. [
π SIMILAR VOLUMES
The effect of salt on the intercalation of acridine dyes and DNA is rather well explained by the Gouy-Chapman double-layer theory as applied to a cylinder model of the DNA-dye complex. The free energy of transfer of a dye ion from the bulk solution to the complex is divided into several parts, one o
## Abstract Controlling the size of condensed DNA particles is a key determinant for their diffusion __in vivo__ as well as for gene delivery to target cells. Towards this goal, DNA molecules were compacted individually by cationic thiolβdetergents into discrete nanometric entities. These particles