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Inhibition and affinity chromatography of chicken lung angiotensin I-converting enzyme with captopril

✍ Scribed by M.J. Polanco; M.T. Agapito; J.M. Recio

Book ID
113323993
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
634 KB
Volume
102
Category
Article
ISSN
1096-4959

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A relatively simple procedure is described for purifying human serum angiotensin-converting enzyme. The enzyme was purified 130,000-fold to electrophoretic homogeneity using affinity chromatography as the principal purification step. The ligand was an immobilized competitive inhibitor, D-cysteinyl-L