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Affinity chromatography of angiotensin I-converting enzyme from rabbit lung using hippurylhistidylleucyl-OH

โœ Scribed by Kazutaka Nishimura; Kunio Hiwada; Einosuke Ueda; Tatsuo Kokubo

Book ID
115734222
Publisher
Elsevier Science
Year
1976
Weight
188 KB
Volume
445
Category
Article
ISSN
0005-2744

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Purification of human serum angiotensin
Purification of human serum angiotensin I-converting enzyme by affinity chromatography
โœ Robert B. Harris; John T. Ohlsson; Irwin B. Wilson ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 742 KB

A relatively simple procedure is described for purifying human serum angiotensin-converting enzyme. The enzyme was purified 130,000-fold to electrophoretic homogeneity using affinity chromatography as the principal purification step. The ligand was an immobilized competitive inhibitor, D-cysteinyl-L