Inheritance and alteration of genome methylation in F1 hybrid rice

✍ Scribed by Tomoko Takamiya; Saeko Hosobuchi; Tomotsugu Noguchi; Kenji Asai; Eiji Nakamura; Yoshiki Habu; Andrew H. Paterson; Hiroshi Iijima; Yasufumi Murakami; Hisato Okuizumi

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 228 KB
• Volume: 29
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.200700784

✦ Synopsis

Abstract

We analyzed the inheritance of DNA methylation in the first filial generation(F1) hybrid of Oryza sativa L. (“Nipponbare”דKasalath”) by restriction landmark genome scanning (RLGS). Most parental RLGS spots were found in the F1, but eight spots (4%) showed abnormal inheritance: seven of the eight spots were missing in the F1, and one was newly detected in the F1. Here we show demethylation at restriction enzyme sites in the F1. We also found a candidate site of stable heterozygous methylation in the genome. These results show the applicability of the RLGS method for analysis of the inheritance and alteration of methylation in F1 hybrid plants.