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Influences of metal ions on the reaction of amino and imino acids with fluorogenic reagents

✍ Scribed by Hiroshi Miyano; Toshimasa Toyo'oka; Kazuhiro Imai; Terumi Nakajima

Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
456 KB
Volume
150
Category
Article
ISSN
0003-2697

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✦ Synopsis

When a twofold excess of metal ions (CL?, Ni*+, Zn*+, Mg*+, Ca*+, Fe'+, and A?+) was added to a reaction medium of amino acids (l-50 pM each) with the fluorogenic reagents NBD-F (4-fluoro-7-nitrobenzo-2-oxa-1,3-diazole), DNS-Cl (5N,Ndimethylaminonaphthalenesulfonyl chloride), OPA (o-phthalaldehyde), and fluorescamine (4-phenylspiro[furan-2(3H),l'-phthalanl-3,3'-dione), a reduction of derivatization was observed. The effect was especially marked on the reaction with NBD-F. The derivatization of His and Asp with DNS-Cl and fluorescamine and of His with OPA was suppressed by CL? and/or Ni*+. The suppression by the metal ions can be overcome by the addition of EDTA to the reaction medium. For determination of a small amount of amino and imino acids (less than pM) the addition of a chelating reagent to biological samples is recommended before derivatization with these electrophilic reagents. o 1985 Academic PUSS. IIIC.

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