Influence of two-phase system composition on biocatalytic properties ofβ-galactosidase preparations

✍ Scribed by W. Bednarski; A. Kulikowska

• Book ID: 111491074
• Publisher: Versita
• Year: 2007
• Tongue: English
• Weight: 197 KB
• Volume: 61
• Category: Article
• ISSN: 0366-6352
• DOI: 10.2478/s11696-007-0049-9

✦ Synopsis

Abstract

A comparison was made between the catalytic properties of β-galactosidase from Penicillium canescens and Aspergillus oryzae using lactose in two-phase systems of an organic solvent and water. It was shown that the ability of β-galactosidase preparations to synthesize galactooligosaccharides depends on the type of the solvent used (cyclohexane, hexane, toluene) and on its proportion in the two-phase system. The use of cyclohexane in the reaction medium stabilized the activity of β-galactosidase from P. canescens which was immobilized on tosylated cotton cloth, and enabled its multiple use in galactooligosaccharides synthesis.