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Influence of the nature of modifier in the enzymatic activity of chemical modified semipurified lipase from Candida rugosa

✍ Scribed by M. J. Hernáiz; J. M. Sánchez-Montero; J. V. Sinisterra

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
196 KB
Volume
55
Category
Article
ISSN
0006-3592

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✦ Synopsis

Semipurified lipase of Candida rugosa (CRSL) was subjected to chemical modification, and the activities of the modified lipase, in hydrolysis and esterification reactions, were examined. The esterification reactions were carried out in the absence and presence of isooctane. When the enzyme was modified with polyethylene glycol (PEG), two methodologies were studied. The activation of PEG with p-NO 2 -phenylchloroformate gives better biocatalysts than those obtained with cyanuric chloride-PEG. The chemical modification with PEG increases the stability of pure lipases in isooctane at 50°C (extreme conditions). The chemically modified enzymes are useful for biotransformations in organic solvents. In addition the nitration of tyrosines with tetranitromethane was also studied.

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