𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Influence of the medium composition and plasmid combination on the growth of recombinant Escherichia coli JM109 and on the production of the fusion protein EcoRI::SPA

✍ Scribed by Jong Il Rhee; Jens Bode; Juan C Diaz-Ricci; Daniel Poock; Beate Weigel; Gerlinde Kretzmer; Karl Schügerl

Book ID
119524739
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
400 KB
Volume
55
Category
Article
ISSN
0168-1656

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Overproduction of EcoRI and SpA::EcoRI f
Overproduction of EcoRI and SpA::EcoRI fusion protein by Escherichia coli employing the combinations of three different types of multicopy plasmids: Comparison of plasmid stabilities and product concentrations
✍ Xiaoan Wu; Lutz Brandes; Hans E. Maschke; Karl Schügerl 📂 Article 📅 2007 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 381 KB

The copy numbers of the expression plasmids (pRTF309+ and pMTC48), the repression plasmids (pc1857 and pRK248cI) and the protection plasmid (pEcoR4) in recombinant E. coli JM 103 were investigated. In the absence of the protection plasmid, the copy number of the expression plasmid dropped; in its pr

Influence of acetate on the growth of re
Influence of acetate on the growth of recombinant Escherichia coli JM103 and product formation
✍ H. H. Lischke; L. Brandes; Xiaoan Wu; K. Schügerl 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 218 KB

The influence of the acetate addeed to the M9 minimal medium and to the Luria-Bertani medium without and with glucose supplement on the growth of recombinant Escherichia coli J 103 with three different types of multicopy plasmids and on the production of the fusion protein SpA :: EcoRI were investig

Fed-batch cultivation of recombinant esc
Fed-batch cultivation of recombinant escherichia coli JM103 and production of the fusion protein SPA::EcoRI in a 60-L working volume airlift tower loop reactor
✍ Lutz Brandes; Xiaoan Wu; Jens Bode; Jong II Rhee; Karl Schügerl 📂 Article 📅 1993 🏛 John Wiley and Sons 🌐 English ⚖ 852 KB

EcoRI fusion protein was produced by Escherichia coli JM103 carrying the multicopy expression plasmid pMTC48, the multicopy repressor plasmid pRK248, and the multicopy protection plasmid pEcoR4 in a 60-L working volume airlift tower loop reactor on M9 minimal medium with glucose. Cell mass concentra