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Influence of acetate on the growth of recombinant Escherichia coli JM103 and product formation

✍ Scribed by H. H. Lischke; L. Brandes; Xiaoan Wu; K. Schügerl

Publisher
Springer
Year
1993
Tongue
English
Weight
218 KB
Volume
9
Category
Article
ISSN
1615-7605

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✦ Synopsis

The influence of the acetate addeed to the M9 minimal medium and to the Luria-Bertani medium without and with glucose supplement on the growth of recombinant Escherichia coli J 103 with three different types of multicopy plasmids and on the production of the fusion protein SpA :: EcoRI were investigated in shake flasks without and with induction of the gene expression by a temperature shift from 30 ~ to 42 ~ At the beginning of the induction of gene expression concentrated LB-medium was added to the shake flask. Without this supplement of M9 medium no gene expression occurred.

List of symbols

LB

Luria Bertani cultivation medium (

Table 2) M9 cultivation medium (Table 1) P enzym activity [u ml 1] TCC total cell count [106 cells ml 1] # specific growth rate [h-1]

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