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Influence of the matrix on the analysis of small oligoribonucleotides by fast atom bombardment mass spectrometry

✍ Scribed by Marek A. Wolter; Joachim W. Engels; Luca Montanarella; Roberto Tilio; Sergio Facchetti

Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
474 KB
Volume
30
Category
Article
ISSN
1076-5174

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✦ Synopsis

Abstract

The analysis of oligonucleotides by mass spectrometry should give information on both the molecular mass and sequence. Fast atom bombardment mass spectrometry gives both in one step. Until now glycerol, thioglycerol and triethanolamine have been used as matrices for the analysis of oligonucleotides by fast atom bombardment mass spectrometry (FABMS), but there have been no comparative studies for matrix selection for these specific analytes. In this work, the influence of diethanolamine, glycerol, 1‐thioglycerol and triethanolamine on the analysis of unprotected trimer up to heptamer oligoribonucleotides was studied using negative‐ion FABMS on a sector‐type mass spectrometer. Collision‐induced dissociation MS/MS measurements were performed and the fragmentation patterns in MS and MS/MS were compared.

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