Influence of the liquid chromatographic mobile phase on the phase transitions of alkyl-bonded silicas studied by gas chromatography

✍ Scribed by D. Morel; J. Serpinet

• Publisher: Elsevier Science
• Year: 1981
• Tongue: English
• Weight: 398 KB
• Volume: 214
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)98525-x

✦ Synopsis

Using gas chromatography of a test solute, we previously demonstrated a phase transition for a densely grafted C,, silica in the usual temperature range of separations by reversed-phase liquid chromatography'. More recently, Kessaissia et al. found a similar transition at cryogenic temperatures for alcohols chemisorbed at

the surface of silica by the same method'.

In the previous paper, we suggested that the hydrophobic pressure of the solvent could increase the transition temperature and that such a phenomenon would complicate the often discussed problem of retention by reversed-phase liquid chroma-tography_ This paper supports this hypothesis by means of "inverse" gas chromatographic experiments in which the bonded material is covered with different stationary phases.

In addition, the use of a Cl2 alkyl chain in place of a C,, chain leads to more abrupt transitions and sheds some light on the critical influence of the chain length in the chromatographic applications of these materials.