Influence of the porous silica gel structure on the coverage density of a chemically bonded C18 phase for high-performance liquid chromatography

✍ Scribed by Bogusa̵w Buszewski; Dušan Berek; Ján Garaj; Ivan Novák; Zdzisa̵w Suprynowicz

• Publisher: Elsevier Science
• Year: 1988
• Tongue: English
• Weight: 777 KB
• Volume: 446
• Category: Article
• ISSN: 1873-3778
• DOI: 10.1016/s0021-9673(00)94433-9

✦ Synopsis

In high-performance liquid chromatography (HPLC), silica gels are the most often used supports for chemically bonded stationary phases. The influence of the physical (volume and diameter of pores) and chemical (hydroxyl group concentration) structures of various silica gel supports on blocking effectively the binding of silanol groups by monochlorooctadecylsilane was investigated. Using silica gel supports with a pore volume 3 1.5 cm3/g and a mean pore diameter > 14 nm as starting materials, reproducible packings for HPLC having a high coverage density (CITY 3 4 ,umol/m') were obtained. Retention behaviour is discussed in terms of a schematic representation of bonded-phase structure.