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Influence of solvent on the mobility of molecules covalently bound to polystyrene matrixes

✍ Scribed by Regen, Steven L.


Book ID
127329287
Publisher
American Chemical Society
Year
1974
Tongue
English
Weight
270 KB
Volume
96
Category
Article
ISSN
0002-7863

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Covalent immobilization of DNA onto poly
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Carbodiimide-mediated condensation of DNA onto microwells is investigated. DNA is bound onto the microwells by formation of a phosphoramidate bond between the 5' terminal phosphate group and the microwells. Immobilization of 25 to 30 ng DNA per well is obtained. DNA molecules bound covalently at onl