✦ LIBER ✦

Covalent immobilization of DNA onto polystyrene microwells: The molecules are only bound at the 5′ end

✍ Scribed by Søren Richard Rasmussen; Mette Ravn Larsen; Svend Erik Rasmussen

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 482 KB
Volume: 198
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90518-x

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✦ Synopsis

Carbodiimide-mediated condensation of DNA onto microwells is investigated. DNA is bound onto the microwells by formation of a phosphoramidate bond between the 5' terminal phosphate group and the microwells. Immobilization of 25 to 30 ng DNA per well is obtained. DNA molecules bound covalently at only the 5' end are, ideally, perfect for hybridization. The practicability of DNA molecules bound to microwells for hybridization is investigated.