Influence of DT diaphorase on quinone-mediated genotoxicity in human and fish cell lines
โ Scribed by Bruce M. Hasspieler; G. Douglas Haffner; Khosrow Adeli
- Book ID
- 114133456
- Publisher
- Elsevier Science
- Year
- 1996
- Tongue
- English
- Weight
- 544 KB
- Volume
- 360
- Category
- Article
- ISSN
- 0165-1161
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## Abstract The 2โ and 4โhydroxy metabolites of 17ฮฒโestradiol (E2) and estrone (E1) are important for E2โmediated carcinogenesis due to the formation of genotoxic orthoโquinone metabolites. To assess the importance of metabolic conjugation for their genotoxicity, the DNA strandโbreaking activity of
Using 4 human cancer cell lines, 4 tumors xenografted into nude mice, and I I fresh tumor specimens removed at surgery, we investigated the relevance of NAD(P)H:quinone oxidoreductase (DT-diaphorase, DTD) activity (nmoles/min/mg protein) to mitomycin C (MMC)-induced cytotoxicity. In culture cell lin