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Influence of development distance on resolution in thin-layer chromatography

✍ Scribed by Franke, J. P. ;Kruyt, W. ;de Zeeuw, R. A.

Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
531 KB
Volume
6
Category
Article
ISSN
0935-6304

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✦ Synopsis

Abstract

Theoretical equations are given to relate resolution with development distance. Comparative experiments undertaken with quaternary ammonium compounds as test substances and using a selection of basic drugs are presented. As expected, resolution decreases with shorter development distance. However, this effect is less pronounced for compounds with small R~f~ values and when the spot size at the starting point is kept small. It was also shown that, for correctly assessing resolution in relation to development distance, the amounts of material spotted have to be decreased for shorter development distances.

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