Influence of Amino Acid Composition and Phosphorylation on the Ion Yields of Peptides in MALDI-MS
β Scribed by Daiki Asakawa; Shohey Moriguchi; Mitsuo Takayama
- Book ID
- 107700808
- Publisher
- Elsevier Science
- Year
- 2011
- Tongue
- English
- Weight
- 434 KB
- Volume
- 23
- Category
- Article
- ISSN
- 1044-0305
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## Abstract Qualitative and quantitative analysis of postβtranslational protein modifications by mass spectrometry is often hampered by changes in the ionization/detection efficiencies caused by amino acid modifications. This paper reports a comprehensive study of the influence of phosphorylation a
Tile dcgrce of sensitivity arid of resolution of amino acids tliat is possible on cellu1osc powder makes thin-layer chromatograpl~y an ideal system for the analysis of protein hydrolysatcs. Its application to biological fluids 1s frequently unsatisfactory, even wllcn salts have Becky removed, owing