✍ Scribed by Norio Nemoto; John L. Schrag; John D. Ferry; Robert W. Fulton
No coin nor oath required. For personal study only.
The storage and loss shear moduli, G′ and G″, have been measured for dilute solutions of unaggregated and aggregated tobacco mosaic virus samples in glycerol–water mixtures, by the Birnboim–Schrag multiple‐lumped resonator modified for use with aqueous solvents. The frequency range was 100–5800 Hz, the concentration range 0.6–2.1 × 10^−3^ g/ml, and the temperatures 25.0° and 37.8°C. The number‐average and weight‐average molecular weights of the aggregated sample were estimated as 1.4 and 2.0 × 10^8^, respectively, from electron microscopy. The extrapolated intrinsic moduli [G′] and [G″] were compared with the predictions of the Kirkwood–Auer theory for rigid rodlike molecules. For the unaggregated sample, the frequency dependence of [G′] and [G″] agreed well with the theory assuming the intrinsic viscosity to be 27 ml/g, though the asymptotic limit of [G′]M/RT at higher frequencies was slightly larger than the theoretical value of 3/5. For the aggregated sample, the data agreed with theory for rigid rods as modified to account for molecular‐weight distribution.
📜 SIMILAR VOLUMES
## Abstract The storage and loss shear moduli, __G__′ and __G__″, have been measured for dilute solutions of bovine fibrinogen in 68% aqueous glycerol and of intermediate fibrin polymer in 6__M__ hexamethylene glycol, by the Birnboim‐Schrag multiple‐lumped resonator apparatus with titanium alloy re
Continuous electrophoresis on filter paper can be a useful technique in the preparation and purification of proteins . It differs significantly from either boundary electrophoresis or zone electrophoresis on filter paper in that it is principally a preparative rather than an analytical tool . In an