β Scribed by Milton Zaitlin
No coin nor oath required. For personal study only.
Continuous electrophoresis on filter paper can be a useful technique in the preparation and purification of proteins . It differs significantly from either boundary electrophoresis or zone electrophoresis on filter paper in that it is principally a preparative rather than an analytical tool . In an earlier short communication1 the reisolation of the individual strains from a synthetic mixture of two strains of purified tobaccc mosaic virus (TMV) was reported . The separation of the two strains was a complete one as the biological integrity of each strain was regained after continuous electrophoresis . This paper extends the description of the electrophoresis technique and its effect on some biological and physical properties of the virus . In addition, the feasibility of purifying TMV without resorting to either chemical purification or high speed centrifugation has been investigated, and the purification of a low molecular weight protein associated with TMV infection is reported .
KARLER 2 , in a brief abstract, has reported the isolation of poliomyelitis virus directly from its culture medium by continuous electrophoresis . He has also examinec TMV, but no details are given .
. MATERIALS AND METHODS
Inoculum of Strains U>; (common) and U2 (mild mosaic) of T11ZV was obtained fron Dr . S . G . WILDMAN . The origins and symptoms produced on tobacco plants arc reported in detail by SIEGEL AND WILDM:\N3 . The virus was purified from infectec Nicotiana tabacuin var . Turkish Samsun plants in a manner analogous to that de scribed by SI> GE_L AND <VILDMAN 3, but using water or dilute phosphate buffer rathe than cacodylic acid buffer as the grinding medium . Generally, two or sometimes three cycles of high and low speed centrifugation were adequate to give a preparatioi " Address after July
π SIMILAR VOLUMES
## Abstract The storage and loss shear moduli, __G__β² and __G__β³, have been measured for dilute solutions of unaggregated and aggregated tobacco mosaic virus samples in glycerolβwater mixtures, by the BirnboimβSchrag multipleβlumped resonator modified for use with aqueous solvents. The frequency ra
## Abstract A tentative and phenomenological analysis of negative electric birefringence, which has often been observed as an anomalous birefringence phenomenon in a concentrated solution of rodlike macromolecules, is presented. Tobacco mosaic virus (TMV) was used as a typical example for the inves