Inexpensive, semi-automated system for measuring mechanical properties of soft tissues

✍ Scribed by Alexander, David E.; Ratzlaff, Kenneth L.; Roggero, Ricardo J.; Hsieh, Jennifer S.-Y.

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 124 KB
• Volume: 284
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/(sici)1097-010x(19990901)284:4<374::aid-jez3>3.0.co;2-q

✦ Synopsis

Stiffness and strength are important properties of many tissues, but standard material-testing equipment is expensive, often ill-suited for testing soft tissues, and rarely accessible to biologists. We describe a system built around a microcomputer and an electronic balance which is particularly well-suited for measuring stress and strain in small samples of soft tissue. We use a discarded floppy disk drive as a linear actuator to strain the sample, while an electronic balance measures the tension (used to calculate stress). We give an algorithm for a program to drive a microcomputer which controls the floppy disk drive via its parallel port and records the balance measurements via its serial port. We used this system to obtain stress-strain curves from a sample of latex rubber and a sample of soft insect cuticle. Three tests of the rubber sample gave nearly identical results, with smooth, J-shaped stress-strain curves. The stress-strain curves gave a modulus elasticity value of 1.72 MPa over the steep, straight region, well within the range for natural latex rubber. We also tested a sample of abdominal cuticle from a caterpillar (Manduca sexta). The caterpillar cuticle had a J-shaped stress-strain curve with a modulus of elasticity of 2.11 MPa over the steep part of the curve.