New soluble chromogenic substrates were prepared for specific and rapid assays of endo-l,4-/3-xylanases and endo-1 ,4+glucanases. A soluble beechwood 4-0-methyl-D-glucurono-D-xylan was dyed with Remazol brilliant blue R, and hydroxyethylcellulos was coupled to Ostazin brilliant red H-3B. The assays
Induction of the Synthesis of Endo-1,4-β-xylanase and β-Galactosidase in Original and Recombinant Strains of the FungusPenicillium canescens
✍ Scribed by E. A. Vavilova; Yu. P. Vinetskii
- Book ID
- 110421345
- Publisher
- SP MAIK Nauka/Interperiodica
- Year
- 2003
- Tongue
- English
- Weight
- 37 KB
- Volume
- 39
- Category
- Article
- ISSN
- 0003-6838
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The title trisaccharide-serine conjugate 1 constituting the linkage region between glycoaminoglycaa and I~otein in proteoglycan, was synthesized via a Ixisacchm~k p-nitrophenyl (PNP) glycoside prepared by stepwise enzymatic transglycosidation to XyI-PNP with a ~-D-galactosidase. In the second tnmsgl
+ 4)-D-GlcpNAc) was synthesized regioselectively with the aid of the transglycosylation activity of P-galactosidase isolated from Diplococcus pneumoniae using p-nitrophenyl P-o-galactopyranoside as the donor. Also, transglycosylation of the sialyl group in an ~(2 + g&linked sialic acid dimer or p-ni