Induction of T4 DNA ligase in a recombinant strain of Escherichia coli
โ Scribed by Gordon K. Whitney; Bernard R. Glick; Campbell W. Robinson
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 741 KB
- Volume
- 33
- Category
- Article
- ISSN
- 0006-3592
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The productivity of Escherichia coli as a producer of recombinant proteins is affected by its metabolic properties, especially by acetate production. Two commercially used E. coli strains, BL21 (DE3) and JM109, differ significantly in their acetate production during batch fermentation at high initia
The production of foreign proteins at high yields represents a severe metabolic stress for Escherichia coli cells. In many cases, induction of protein synthesis results in rapid exhaustion of the cellular energy and metabolic precursors and thus in cell death. Therefore, sustained production of fore