We devised recently a method to trap intact isolated chloroplasts on a solid support consisting of membrane filters made of cellulose nitrate (Cerovi6 et al., 1987, Plant Physiol. 84, 1249-1251). The addition of alkaline phosphatase to the reaction medium enabled continuous photosynthesis by spinach
Induction of oscillations in chlorophyll fluorescence by re-illumination of intact isolated pea chloroplasts
✍ Scribed by Sonja Veljović-Jovanović; Zoran G. Cerović
- Publisher
- Springer-Verlag
- Year
- 1991
- Tongue
- English
- Weight
- 414 KB
- Volume
- 185
- Category
- Article
- ISSN
- 0032-0935
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✦ Synopsis
Oscillations in chlorophyll fluorescence yield were observed upon re-illumination of intact isolated pea (Pisum sativum L.) chloroplasts that had attained their maximal rate of photosynthesis and had spent a short period in darkness. The oscillations depended on the length of the previous dark period, the length of previous illumination, and the reaction temperature. This finding confirms the presence of an "oscillatory center" in the chloroplasts temselves.
📜 SIMILAR VOLUMES
Simultaneous "ripples" (sudden changes in rate) in COs dependent 02 evolution and associated chlorophyU a fluorescence were followed in isolated, largely intact, spinach chloroplasts. These ripples could only be observed under conditions in which the supply of inorganic phosphate was limiting. This