Induction of mitotic gene conversion in Saccharomyces cerevisiae by N-nitrosated pesticides
โ Scribed by D. Siebert; G. Eisenbrand
- Book ID
- 116036849
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 312 KB
- Volume
- 22
- Category
- Article
- ISSN
- 0027-5107
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Gene conversion of large DNA heterologous fragments has been shown to take place efficiently in Saccharomyces cerevisiae. It has been found that a 2.6 kb LEU2 DNA fragment in a multicopy plasmid was replaced by a 3.1 kb PGI1 chromosomal DNA fragment, when both fragments were flanked by homologous DN
In the Saccharomyces cerevisiae mitotic cycle, the timing of radiation-induced gene conversion has been studied using thermosensitive cell division cycle mutants. The cells were found to perform conversion at different G1 or post-replication steps. A lower yield in induction is found during the G2 p