Gene conversion of large DNA heterologous fragments has been shown to take place efficiently in Saccharomyces cerevisiae. It has been found that a 2.6 kb LEU2 DNA fragment in a multicopy plasmid was replaced by a 3.1 kb PGI1 chromosomal DNA fragment, when both fragments were flanked by homologous DN
Gene conversion at different points in the mitotic cycle of Saccharomyces cerevisiae
โ Scribed by Fabre, F. ;Boulet, A. ;Roman, H.
- Publisher
- Springer
- Year
- 1984
- Tongue
- English
- Weight
- 511 KB
- Volume
- 195
- Category
- Article
- ISSN
- 0026-8925
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โฆ Synopsis
In the Saccharomyces cerevisiae mitotic cycle, the timing of radiation-induced gene conversion has been studied using thermosensitive cell division cycle mutants. The cells were found to perform conversion at different G1 or post-replication steps. A lower yield in induction is found during the G2 phase and is explained by the competition for recombinational repair between sister chromatids and homologous chromosomes. The results are discussed in relation to repair.
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