Induction of metallothionein by zinc inlethal milkmutant mice

✍ Scribed by John E. Piletz; Harvey R. Herschman

• Book ID: 104787401
• Publisher: Springer
• Year: 1982
• Tongue: English
• Weight: 645 KB
• Volume: 20
• Category: Article
• ISSN: 0006-2928
• DOI: 10.1007/bf00498945

✦ Synopsis

Adult lethal milk (lm/lm) mutant mice display increased induction of hepatic metallothionein synthesis compared to wild-type mice following the subcutaneous injection of 40 #tool ZnCl2/kg mouse. At this zinc dose the rate of incorporation of FSS]cysteine into hepatic metallothionein in adult (lO0to 230-day-old) lm/lm mice was approximately 2.4-fold greater than the rate of incorporation of isotope in wild-type animals. At a higher zinc dose (160 #mol ZnCl2/kg) the incorporation of FSS]cysteine into hepatic metallothionein was similar in lm/lm and wild-type mice. The altered dose-response to zinc administration was not due to a change in hepatic zinc, copper, or manganese levels, to a difference in 65Zn uptake, or to an alteration in 65Zn bound to differential centrifugation fractions of adult lm/lm liver. 65Zn bound to hepatic metallothionein was, however, increased in aging lm/lm mice with symptomatic skin lesions.