𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Induction of meiosis by injection of heterologous protein kinase and phosphorylase kinase inXenopus laevis oocytes

✍ Scribed by Moreau, Marc ;Guerrier, Pierre ;Doree, Marcel

Book ID
102891298
Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
612 KB
Volume
197
Category
Article
ISSN
0022-104X

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Meiosis reinitiation has been triggered by injection of beef heart protein kinase or rabbit phosphorylase kinase into Xenopus laevis oocytes. Successful injections are followed by germinal vesicle breakdown, chromosome condensation, formation of a normal meiotic spindle, and appearance of an amplifiable maturation promoting factor. Meiosis reinitiation does not occur when the enzymes are introduced into the oocytes simultaneously with EGTA or after pretreatment with cycloheximide. Antipain, an antiprotease which abolishes the response of oocytes to progesterone, does not suppress the meiosis reinitiation induced by injection of protein kinase or phosphorylase kinase.

πŸ“œ SIMILAR VOLUMES

The induction of pyruvate kinase synthes
The induction of pyruvate kinase synthesis by heat shock inXenopus laevis embryos
✍ Marsden, M. ;Nickells, R. W. ;Kapoor, M. ;Browder, L. W. πŸ“‚ Article πŸ“… 1993 πŸ› John Wiley and Sons 🌐 English βš– 689 KB

## Abstract Heat‐shocked __Xenopus__ embryos have an unusually complex heat shock response. The dominant heat shock protein (Hsp) has a relative molecular mass (__M__~r~) of 62,000 D (Hsp62). Affinity‐purified IgGs against the glycolytic enzyme pyruvate kinase (PK; EC 2.7.1.40) specifically immunop