𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Induction of lysosomal enzymes in contact inhibited 3T3 cells

✍ Scribed by A. Horvat; G. Ács

Publisher
John Wiley and Sons
Year
1974
Tongue
English
Weight
839 KB
Volume
83
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The pattern of changing activities of three lysosomal enzymes, N‐acetyl‐β‐D‐glucosaminidase, aryl sulfatase, and DNase II, and that of DNA polymerase was followed in homogenates of 3T3 cells during the logarithmic phase of growth and in stationary cultures. The change in activities of the polymerase and the lysosomal enzymes is antiparallel. DNA polymerase exhibits highest activity in growing cultures, and shows a three‐fold decline of the specific activity in stationary cultures. The lysosomal enzymes show a very marked increase in their specific activity after density saturation is reached, which can be prevented by the addition of cycloheximide. Colcemid added to logarithmically growing cultures also causes an increase in the specific activities of lysosomal enzymes.

📜 SIMILAR VOLUMES

Contact inhibition of speed in 3T3 and i
Contact inhibition of speed in 3T3 and its independence from postconfluence inhibition of cell division
✍ Eric Martz 📂 Article 📅 1973 🏛 John Wiley and Sons 🌐 English ⚖ 827 KB

## Abstract The average speed of nuclear translocation of 3T3 cells, recorded in a time‐lapse film of a perfused culture, was negatively correlated with the number of contacting cells, and, to a lesser degree, with the amount of a cell's perimeter in contact with other cells. When a cell was in con

Cell-cell contact and growth regulation
Cell-cell contact and growth regulation of pinocytosis in 3T3 cells
✍ Davies, Peter F. 📂 Article 📅 1980 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 423 KB

## Abstract In sub‐confluent cultures of Balb/c‐3T3 cells, pinocytosis rates were increased after exposure to specific growth factors (serum; platelet‐derived growth factor, PDGF; epidermal growth factor, EGF). Conversely, as cells became growth‐inhibited with increasing culture density, there was

Inhibition of sugar uptake in adenosine-
Inhibition of sugar uptake in adenosine-treated 3T3 cells
✍ David W. Barnes; Verona T. Brown; Sidney P. Colowick 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 688 KB

## Abstract Addition of 5 to 250 micromolar adenosine to the culture medium resulted in a 30–80% inhibition of the rate of uptake of 2‐deoxyglucose or 3–0‐methylglucose by sparse or confluent 3T3 cells within three hours. The inhibition of deoxyglucose uptake could be reversed partially by changing

Age-dependent decrease of growth respons
Age-dependent decrease of growth responsiveness in density inhibited 3T3 cell populations and their interactions with SV 40-3T3 cells
✍ Jürgen van Der Bosch 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 754 KB

## Abstract An aging process has been detected in stationary 3T3 cell cultures, especially in the presence of plasma‐derived serum (PDS) from adult bulls. It leads to irreversible conversion of an increasing percentage of initially responsive cells of a stationary population into cells unresponsive

Induction of osteoblast differentiation
Induction of osteoblast differentiation indices by statins in MC3T3-E1 cells
✍ Toyonobu Maeda; Ayako Matsunuma; Izuru Kurahashi; Toru Yanagawa; Hiroshi Yoshida 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 261 KB 👁 1 views

## Abstract Statins inhibit 3‐hydroxy‐3‐methylglutaryl‐coenzyme A (HMG‐CoA) reductase, which catalyzes conversion of HMG‐CoA to mevalonate, a rate‐limiting step in cholesterol synthesis. The present study was undertaken to understand the events of osteoblast differentiation induced by statins. Simv