Induction of human cytotoxic T lymphocytes specific for prostate-specific antigen

✍ Scribed by Xue, Bao-Hua; Zhang, Yong; Sosman, Jeffrey A.; Peace, David J.

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 255 KB
• Volume: 30
• Category: Article
• ISSN: 0270-4137
• DOI: 10.1002/(sici)1097-0045(19970201)30:2<73::aid-pros1>3.0.co;2-l

✦ Synopsis

Prostate-specific antigen (PSA), a tissue-specific protein expressed by most adenocarcinomas of the prostate, might be a useful target for T-cell-mediated immunotherapy of prostate cancers. The current study examined whether it is possible to elicit human cytotoxic T lymphocytes (CTL) with specificity for PSA. A synthetic nonamer peptide, corresponding to residues 146-154 of PSA and containing a canonical HLA-A2-binding motif, was shown to stabilize the expression of HLA-A2 on the T2 antigen-processing mutant cell line. Repeated in vitro stimulation of peripheral blood lymphocytes from a normal HLA-A2 + donor induced CTL with specificity for the PSA 146-154 peptide. The peptide-induced CTL expressed the CD4 -CD8 + cell surface phenotype and were restricted by HLA-A2. A large portion of patients with prostate cancer express the HLA-A2 phenotype, implying that many prostate cancers might be targeted by HLA-A2-restricted CTL with specificity for the PSA 146-154 epitope.