Induction of Foxp3 expression in T cells by cellulose acetate beads in vitro

✍ Scribed by Fumie Jimma; Yuji Takeda; Kenta Kaneda; Ichiro Wakabayashi

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 228 KB
• Volume: 25
• Category: Article
• ISSN: 0733-2459
• DOI: 10.1002/jca.20243

✦ Synopsis

Abstract

Gene expression of transforming growth factor‐β (TGF‐β) is needed to induce expression of transcription factor forkhead box P3 (Foxp3), which is required for the development and function of regulatory T (Treg) cells. The number of circulating Treg cells and the level of Foxp3 expression increase during granulocyte and monocyte apheresis (GMA), a useful therapy for ulcerative colitis. However, the mechanism underlying GMA‐induced Foxp3 expression is unknown. We found that the level of TGF‐β mRNA in peripheral blood mononuclear cells (PBMCs) was augmented just after treatment of peripheral blood with a GMA carrier, cellulose acetate beads, in vitro and that Foxp3 expression in PBMCs increased after culturing these cells for 5 days after the treatment. The augmentation of TGF‐β expression was observed in CD3^−^ PBMCs but not in CD3^+^ T cells. Furthermore, the increase in Foxp3 expression in T cells depended on co‐culture with CD3^−^ PBMCs. We conclude that cellulose acetate beads have an ability to induce Foxp3 expression in peripheral blood T cells via augmentation of TGF‐β expression in CD3^−^ PBMCs. J. Clin. Apheresis 25:216–222, 2010. © 2010 Wiley‐Liss, Inc.