Induction of fibroblast proliferation by interleukin-1 derived from human monocytic leukemia cells

✍ Scribed by Arnold E. Postlethwaite; Lawrence B. Lachman; Andrew H. Kang

• Publisher: John Wiley and Sons
• Year: 1984
• Tongue: English
• Weight: 677 KB
• Volume: 27
• Category: Article
• ISSN: 0004-3591
• DOI: 10.1002/art.1780270905

✦ Synopsis

Human interleukin-1 (IL-l), free of contaminating lymphokines, was isolated from cultures of purified monoblasts from a patient with acute monocytic leukemia. Partially purified IL-1 (diafiltration, ultrafiltration, and isoelectric focusing) stimulated proliferation of subconfluent human fibroblasts in vitro. Further purification of IL-1 by high-resolution gel filtration-and anion exchangehigh performance liquid chromatography revealed that fibroblast proliferation activity could not be separated from IL-1 activity (thymocyte proliferation), suggesting that both activities are the properties of a single molecule. Fibroblasts and thymocytes exhibited a similar sensitivity to the proliferative effects of IL-1. These findings suggest that macrophages participating in inflammatory reactions in vivo might release IL-1, which could function to expand fibroblast populations at sites of inflammatory reactions, by acting as a fibroblast growth factor.

Interleukin-1 (IL-l), or lymphocyte activating factor (LAF), is a monokine released in vitro by cultured monocytes or macrophages. Interleukin-1 acts on a variety of target cells in a genetically From the