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Induction of DNA strand breaks by trihalomethanes in primary human lung epithelial cells

✍ Scribed by Stefano Landi; Alessio Naccarati; Matthew K. Ross; Nancy M. Hanley; Lisa Dailey; Robert B. Devlin; Marie Vasquez; Rex A. Pegram; David M. DeMarini

Book ID
117767135
Publisher
Elsevier Science
Year
2003
Tongue
English
Weight
249 KB
Volume
538
Category
Article
ISSN
1383-5718

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In vitro exposure of normal human diploid fibroblasts (strain VH-10) to ethylene oxide (EtO) induced DNA strand breaks in the dose range of 2.5-30 mMh of EtO. Alkaline DNA unwinding (ADU), neutral filter elution (NFE), pulsed field gel electrophoresis (PFGE), and the comet assay were used to measure