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Induction of DNA strand breaks by reduced nitroimidazoles: Implications for DNA base damage

✍ Scribed by Andrew R.J. Silver; Shona S. Mcneil; Peter O'Neill; Terence C. Jenkins; Israr Ahmed


Book ID
115778683
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
904 KB
Volume
35
Category
Article
ISSN
0006-2952

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In vitro exposure of normal human diploid fibroblasts (strain VH-10) to ethylene oxide (EtO) induced DNA strand breaks in the dose range of 2.5-30 mMh of EtO. Alkaline DNA unwinding (ADU), neutral filter elution (NFE), pulsed field gel electrophoresis (PFGE), and the comet assay were used to measure